# Which of the following contains most aggressive bacteria penetrating connective tissue and resulting in bone destruction?
A. Subgingival unattached plaque
B. Subgingival tooth attached plaque
C. Subgingival attached plaque
D. Supragingival plaque
The correct answer is A. Subgingival unattached plaque.
The total width of the plaque-free zone varies according to the type of tooth (i.e., it is wider in the molars than in the incisors) and the depth of the pocket (i.e., it is narrower in deeper pockets). It is important to remember that the term plaque-free zone refers only to attached plaque, because unattached plaque contains a variety of gram-positive and gram-negative morphotypes, including cocci, rods, filaments, fusiforms, and spirochetes. The most apical zone contains predominantly gram-negative rods and cocci.
Reference: Carranza's Clinical Periodontology, 12th Edition, Page no: 285.
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